Xiuchun (Cindy) Tian

Professor

Adjunct Faculty Member of MCB & Member of the UConn Stem Cell Institute


Contact


   Phone: (860) 486-9087

   Email: Xiuchun.Tian@UConn.edu

   Office: ABL Building, Room 220D, Unit-4163

   Lab: ABL Building, Room 231

   Lab Phone: (860) 486-6912

 

Background

Education

  • Ph.D. (Physiology), Cornell University, 1995
  • M.S. (Physiology), Cornell University, 1989
  • B.S. (Animal Science), China Agricultural University, 1985

 
Dr. Tian obtained her MS and PhD from Cornell University where she conducted research in early pregnancy recognition and regulation of steroidogenesis in ovarian tissues. She then received post-doctoral trainings as a recipient of a National Research Service Award from NIH in developmental genetics and molecular embryology at Cornell University and University of Connecticut.

 

Academics

Area of Interest

The research interest of Dr. X. Cindy Tian is to understand the process of gene reprogramming by somatic nuclear transfer. Since the creation of Dolly, the first mammal cloned from an adult differentiated somatic cell, a long-held dogma in developmental biology, that the process of genes inactivation during development and differentiation was irreversible, was revolutionized. We now believe that inactivated genes can be reactivated by the somatic cloning technology and are capable to direct development of a new individual. The process of gene reactivation by somatic nuclear transfer is called nuclear or gene reprogramming. Nuclear reprogramming is the fundamental basis for tissue regeneration by the stem cell technology. The high rates of developmental abnormality of somatic clones, however, point to the fact that the nuclear reprogramming process is incomplete and imperfect at this time. Understanding the mechanisms regulating the nuclear/gene reprogramming events will lead to technologies to targeted re-differentiation of cells for tissue regeneration.

Classes Taught

ANSC 3323 Animal Embryology and Biotechnology
ANSC 3324W Scientific Writing in Embryo Biotechnology
ANSC 3621 Animal Biotechnology Laboratory
ANSC 5621 Frontiers in Animal Embryo Biotechnology

Awards & Achievements

    • 2015: Member of American Association for the Advancement of Science (AAAS)
    • 2015: Editorial Board member, Scientific Reports
    • 2015: Editorial Board member, Journal of Reproduction and Development
    • 2011: Editorial Board member, PLoS ONE.
    • 2010: Section Editor, BMC Developmental Biology
    • 2008: Program co-chair, International Embryo Transfer Society 2009 Annual meeting, San Diego, TX
    • 2007-2015: Associate editor, BMC Research Notes
    • 2006: CANR Research Excellence Award

Current Positions of Former Graduate Students

Dr. Ching-Chien Chang:         Embryologist, Reproductive Biology Associates, Atlanta, Georgia

Dr. Carol Curchoe George:     President and CEO of 32ATPs

Dr. Le Jiang:                         Staff Scientist, US Naval Medical Research Center

Dr. Chih-Jen (Lance) Lin:        Faculty member, Medical Research Council, UK

Dr. Sadie (Smith) Marjani:      Associate Professor, Central Connecticut State University

Dr. Joonghoon Park:              Assistant Professor, Seoul National University

Dr. Yong(Young) Tang:           Assistant Professor, University of Connecticut

Dr. Shouquan (Stan) Zhang:   Professor and Associate Dean, South China Agricultural University, China

Dr. Zongliang (Carl) Jiang:      Assistant Professor, Louisiana State University

 

Publications

High Impact Primary Publications

1. Tang Y, Luo Y, Jiang Z, Ma Y, Kim C, Lin CJ, Amano T, Park J, Amano M, Carter MG, Kish S, and Tian XC*. 2012. Jak/Stat3 Signaling Promotes Somatic Cell Reprogramming by Epigenetic Regulation. Stem Cells 2012 Dec;30(12):2645-56. doi: 10.1002/stem.1225 (April 2012).

2. Inoue K, Kohda T, Sugimoto M, Sado T, Ogonuki N, Matoba S, Shiura H, Ikeda R, Mochida K, Fujii T, Sawai K, Otte AP, Tian XC, Yang X, Ishino F, Abe K, Ogura A. 2010. Impeding Xist Expression from the Active X Chromosome Improves Mouse Somatic Cell Nuclear Transfer. Science 330:496-9. Epub 2010 Sep 16.

3. Mansouri-Attia N, Sandra O, Aubert J, Degrelle S, Everts RE, Giraud-Delville C, Heyman Y, Galio L, Hue I, Yang X, Tian XC, Lewin HA, Renard JP. 2009. Endometrium as an early sensor of in vitro embryo manipulation technologies.  Proc Natl Acad Sci USA 106:5687-92. Epub 2009 Mar 18.

4. Yang X, Tian XC, Kubota C, Page R, Xu J, Cibelli J, Seidel G Jr. 2007. Risk assessment of meat and milk from cloned animals. Nature Biotech 25:79-83.

5. Yang X, Smith SL, Tian XC, Lewin HA, Renard JP, Wakayama T. 2007.  Nuclear reprogramming of cloned embryos and its implications for therapeutic cloning. Nature Gen 39:295-302.

6. Sung L-Y, Gao S, Shen H, Yu H, Song Y, Smith SL, Chang C-C, Inoue K, Kuo K, Lian J, Li A, Tian XC, Tuck DP, Weissman SM, Cheng T, Yang X. 2006. Differentiated cells are more efficient than adult stem cells for cloning by somatic cell nuclear transfer. . Nature Genetics 38:1323-8.

7. Smith SL, Everts RE, Tian XC*, Du F, Sung L-Y, Rodrigues-Zas S, Jeong B-S, Renard JP, Lewin HA, Yang X. 2005. Global Gene Expression Profiles of Cloned Blastocysts Reveal Significant Nuclear Reprogramming. Proc Natl Acad Sci USA 102:17582-7.

8. Tian, XC*, Kubota C, Sakashita K, Izaike Y, Okano R, Tabara N, Curchoe C, Jacob L, Zhang Y, Smith S, Bormann C, Andrew S, Yang X. 2005. Meat and Milk Compositions of Bovine Clones Compared with Matched Controls. Proc Natl Acad Sci USA 102: 6261-6.

9. Kubota C, Tian XC, Yang X. 2004. Differential fertility in second and third generation clones of a prize breeding bull. Nature Biotechnology 22: 693-694.

10. Xue F, Tian XC#, Kubota C, Du F, Taneja M, Dinnyes A, Dai Y, Pereira LV, Yang X. 2002. Aberrant X-Chromosome inactivation in deceased cattle derived from somatic cloning. Nature Genetics 31:216-220.

11. Tian XC*, Xu J, Yang X. 2000. Normal Telomere Lengths Found in Cloned Cattle. Nature Genetics 26:272-273.

Other full-length, peer-reviewed, primary publications

12.  Wang, L., Huang, D., Jiang, Z., Luo, Y., Carol, B., Zhang, M., Tian, X., & Tang, Y. (2017). Akt3 Is Responsible for Survival and Proliferation of Embryonic Stem Cells. Biology Open 2017 : doi: 10.1242/bio.024505

13.  Tribulo, P., Moss, J. I., Ozawa, M., Jiang, Z., Tian, X., & Hansen, P. J. (2017). Developmental Changes in Canonical WNT Signaling in the Preimplantation Bovine Embryo. 143(4), 405-419. 10.1530

14. Sun JW, Jiang J, Tian X, and Bi J. 2016. A Cross-Species Bi-Clustering Approach to Identifying Conserved Co-regulated Genes. Bioinformatics (accepted 4/20/2016).

15. Park JH, Park JW, Oh H, Maria FS, Kang J, and Tian XC*. 2016. Gene-specific assessment of guanine oxidation as an epigenetic modulator for cardiac specification of mouse embryonic stem cells. PLoS ONE (accepted 5/5/2016).

16. Jiang Z, Harrington P, Zhang M, Marjani SL, Park J, Kuo L, Pribenszky C, Tian XC*. 2016. Effects of High Hydrostatic Pressure on Expression Profiles of In Vitro Produced Vitrified Bovine Blastocysts. Scientific Reports 6:21215. doi: 10.1038/srep21215.

17. Jiang Z, Dong H, Zheng X, Donovan DM, Chen J, Tian XC*. 2015. mRNA Levels of Imprinted Genes in Bovine In Vivo Oocytes, Embryos and Cross Species Comparisons in Humans, Mice and Pigs. Scientific Reports 5:17898. doi: 10.1038/srep17898.

18. Park JP, Lai L, Samuel MS, Wax D, Prather RS, Tian XC*. 2015. Disruption of mitochondria-to-nucleus interaction in deceased cloned piglets. PLoS ONE (submitted on Feb 28, 2015, accepted May 12, 2015).

19. Shi R, Bai Y, Li S, Wei H, Zhang X, Li L, Tian XC, Jiang Q, Wang C, Qin L, Cai J & Zhang S. 2014. Characteristics of spermatogonial stem cells derived from neonatal porcine testis. Andrologia. Published online: 23 SEP 2014. DOI: 10.1111/and.12327

20. Jiang Z, Tang Y, Zhao X, Donovan DM, XC Tian*. 2015. Knockdown Brm and Baf170, Components of Chromatin Remodeling Complex, Facilitates Reprogramming of Somatic Cells. Stem Cell and Development (submitted on Feb 17, 2015, accepted with revision).

21. Lin, CJ; Amano, T; Tang Y; TianXC*. 2014. Improved derivation efficiency and pluripotency of stem cells from the refractory inbred C57BL/6 mouse strain by small molecules. PLoSONE 9(9): e106916 published online Sept 11, 2014.

22. Jiang Z, Sun J, Dong H, Luo O, Zheng X, Obergfell C, Tang Y, Bi J, O’Neill R, Ruan Y, Chen J, Tian XC*. 2014. Complete Transcriptional Profiles of Bovine In Vivo Pre-implantation Development. BMC Genomics 15:756; published online Sept 4, 2014.

23. Tang Y, Jiang Z, Luo Y, Zhao X, Wang L, Tian XC*. (2014). Differential Effects of Akt Isoforms on Somatic Cell Reprogramming.  Journal of Cell Science (accepted July, 2014).

24. Yuan Y, Su M, Song Y, Tang Y, Tian XC, Rood D and Lai L. (2014). Tbx1 Modulates Endodermal and Mesodermal Differentiation from Mouse Induced Pluripotent Stem cells. Stem Cells Dev. 23(13):1491-500.

25. Krueger WH, Tanacivjic B, Norris C, Tian XC, and Rasmussen TP. 2013. Oct4 Promoter Activity in Stem Cells obtained through Somatic Reprogramming. Cell Reprogram 15(2):151-8.

26. Li Y; Pal R; Sung LY; Feng H; Miao W; Cheng SY; Tian C, Cheng T. 2012. An opposite effect of the CDK inhibitor, p18INK4c on embryonic stem cells compared with tumor and adult stem cells. PLoS ONE 7(9): e45212. doi:10.1371/journal.pone.0045212 (May 18, 2012).

27. Degrelle SA, Jaffrezic F, Campion E, Le Cao K-A, Lebourhis D, Richard C, Rodde N, Fleurot R, Everts RE, Lecardonel J, Heyman Y, Vignon X, Yang X, Tian XC, Lewin HA, Renard JP and Hue I. 2012. Uncoupled embryonic and extra-embryonic tissues compromise blastocyst development after somatic cell nuclear transfer. PLoS One 7(6) e38309 (May 2012).

28. Kim C, Park J, Amano T, Xu RH, L Gu, Carter MG, Tian XC*. 2012. Established preblastocyst- and blastocyst-derived ES cell lines have highly similar gene expression profiles, despite their differing requirements for derivation culture conditions. Cellular Reprogramming 14(1):1-7 (accepted Dec 2011, Epub Jan 18, 2012).

29. Li-Ying Sung, Chien-Hong Chen, Jie Xu, Tzu-An Lin, Hwa-Yun Su, Wei-Fang Chang, Chia-Chia Liu, Yun-Shao Sung, Winston T.K. Cheng, Jifeng Zhang, X. Cindy Tian, Jyh-Cherng Ju, Y. Eugene Chen, Shinn-Chih Wu, and Fuliang Du. 2011. Follicular Oocytes Better Support Development in Rabbit Cloning Than Oviductal Oocytes. Cellular Reprogramming 13(6):503-512 (Epub 2011 Oct 26).

30. Changli Wu, Xinzheng Guo, Fang Wang, Xiaoshuang Li, X Cindy Tian, Li Li, Zhenfang Wu, Shouquan Zhang. 2011. Simulated Microgravity Compromises Mouse Oocyte Maturation by Disrupting Meiotic Spindle Organization and Inducing Cytoplasmic Blebbing. PLoS One 6(7): e22214. Epub 2011 July 13.

31. Tang T, Lin CJ, Tian XC*. 2011. Functionality and transduction condition evaluation of recombinant Klf4 for improved reprogramming of iPS cells. Cellular Reprogramming 13(2):99-112 (April 2011).

32. Jiang L, Marjani SL, Bertolini M, Everts RE, Lewin HA, Anderson GB, Yang X, Tian XC*. 2011. Indistinguishable Transcriptional Profiles Between In Vitro and In Vivo-Produced Bovine Fetuses. Mol Reprod Dev 78(9):642-650 (Jul 8. doi: 10.1002/mrd.21362. [Sept 2011]).

33. Park J, Kim C, Tang Y, Amano T, Lin CJ, Tian XC*. 2011. Reprogramming of mouse fibroblasts to an intermediate state of differentiation by chemical induction. Cell Reprogram. 13(2):121-31 (April 2011).

34. Park J, Lai L, Samuel M, Wax D, Bruno RS, French R, Prather RS, Yang X, Tian XC*. 2011. Altered Gene Expression Profiles in the Brain, Kidney, and Lung of One-Month Old Live Cloned Pigs. Cellular Reprogramming 13(3): 215-223 (June 2011).

35. Degrelle SA, Lê Cao KA, Heyman Y, Everts RE, Campion E, Richard C, Ducroix-Crépy C, Tian XC, Lewin HA, Renard JP, Robert-Granié C, Hue I. 2011. A small set of extra-embryonic genes defines a new landmark for bovine embryo staging. Reproduction 141:79-89. Epub 2010 Oct 6.

36. Chang CC, Lin CJ, Sung LY, Kort KI, Tian XC, Nagy ZP. 2011. Impact of phase transition on the mouse oocyte spindle during vitrification. RMBonline 22:184-91.

37. Lin CJ, Amano T, Zhang J, Chen YE, Tian XC*. 2010. Acceptance of embryonic stem cells by a wide developmental range of mouse tetraploid embryos. Biol Reprod 83:177-84. Epub 2010 Apr 21.

38. Adams HA, Southey BR, Everts RE, Marjani SL, Tian XC, Lewin HA, Rodriguez-Zas SL. 2010. Transferase activity function and system development process are critical in cattle embryo development. Funct Integr Genomics. 2010 Sep 16. [Epub ahead of print].

39. Senatore E, Xu J, Novoa M, Gong G, Lin T, Bella A, Moreno JF, Mannino M, Tian CX, Presicce GA, Wu S, and Du F. 2010. Improved in vitro development of OPU-derived bovine (Bos taurus) embryos by group culture with agarose-embedded helper embryos. Theriogenology 74:1643-1651. Aug 11. [Epub ahead of print] PMID: 20708248.

40. Presicce GA, Xu J, Gong G, Moreno JF, Chaubal S, Xue F, Bella A, Senatore EM, Yang X, Tian XC, Du F. 2010. Oocyte source and hormonal stimulation for in vitro fertilization using sexed spermatozoa in cattle. Vet Med Int. 2010 Sep 5;2011. pii: 145626. PMID: 20885928 [PubMed - in process].

41. Chang CC, Sung LY, Lin CJ, Kort HI, Yang X, Tian XC, Nagy ZP. 2010. The oocyte spindle is preserved by 1,2-propanediol during slow freezing. Fertil Steril 93:1430-9. Epub 2009 Mar 26.

42. Park J, Marjani S, Lai L, Samuel M, Wax D, Davis S, Bruno R, Prather R, Yang X, Tian XC*. 2010. Altered Gene Expression Profiles in the Brain, Kidney, and Lung of Deceased Neonatal Cloned Pigs. Cellular Reprogramming 12:589-97.

43. Hou S, Chen Y, Liang J, Li L, Wu T, Tian XC#, Zhang S. 2010. Developmental Stage Specific Imprinting of IPL in Domestic Pigs (Sus scrofa). J Biomed Biotech 2010: 527539. Epub 2010 Jun 2.

44. Sung LY, Chang CC, Amano T, Lin CC, Amano M, Xu J, Chang WF, Nagy ZP, Yang X, Tian XC*. 2010. Efficient derivation of embryonic stem cells from nuclear transfer and parthenogenetic embryos derived from cryopreserved oocytes. Cellular Reprogramming 12:203-211.

45. Chang CC, Gao S, Sung LY, Corry GN, Ma Y, Nagy ZP, Tian XC#, Rasmussen TP. 2010. Rapid Elimination of the Histone Variant MacroH2A from Somatic Cell Heterochromatin after Nuclear Transfer. Cellular Reprogramming 12:43-53.

46. Gong G, Roach ML, Jiang L, Yang X, Tian XC*. 2010. Culture Conditions and Enzymatic Passaging of Bovine ESC-Like Cells. Cellular Reprogramming 12:151-160.

47. Chang CC, Sung LY, Lin CJ, Kort HI, Yang X, Tian XC, Nagy ZP. 2009. The oocyte spindle is preserved by 1,2-propanediol during slow freezing. Fertil Steril 93:1430-1439. Mar 24. [Epub ahead of print].

48. Mansouri-Attia N, Aubert J, Reinaud P, Giraud-Delville C, Taghouti G, Galio L, Everts RE, Degrelle S, Richard C, Hue I, Yang X, Tian XC, Lewin HA, Renard JP, Sandra O. 2009. Gene expression profiles of bovine caruncular and intercaruncular endometrium at implantation. Physiol Genomics 39:14-27. Epub 2009 Jul 21.

49. Curchoe CL, Zhang S, Yang L, Page R, Tian XC*. 2009. Hypomethylation trends in the intergenic region of the imprinted IGF2 and H19 genes in cloned cattle. Anim Reprod Sci 116:213-25. Epub 2009 Feb 11.

50. Suteevun-Phermthai T, Curchoe CL, Evans AC, Boland E, Rizos D, Fair T, Duffy P, Sung LY, Du F, Chaubal S, Xu J, Wechayant T, Yang X, Lonergan P, Parnpai R, Tian XC*. 2009. Allelic switching of the imprinted IGF2R gene in cloned bovine fetuses and calves. Anim Reprod Sci 116:19-27. Epub 2009 Jan 20.

51. Kim C, Amano T, Park J, Carter MG, Tian X, Yang X. 2009.  Improvement of embryonic stem cell line derivation efficiency with novel medium, glucose concentration, and epigenetic modifications. Cloning Stem Cells 11:89-100.

52. Du F, Xu J, Zhang J, Gao S, Carter MG, He C, Sung LY, Chaubal S, Fissore RA, Tian XC, Yang X, Chen YE. 2009. Beneficial effect of young oocytes for rabbit somatic cell nuclear transfer. Cloning Stem Cells 11:131-40.

53. Smith SL, Everts RE, Sung LY, Du F, Page RL, Henderson B, Rodriguez-Zas SL, Nedambale TL, Renard JP, Lewin HA, Yang X, Tian XC*. 2009. Gene expression profiling of single bovine embryos uncovers significant effects of in vitro maturation, fertilization and culture. Mol Reprod Dev 76:38-47.

54. Jiang L, Lai L, Samuel M, Prather RS, Yang X, Tian XC*. 2008. Expression of X-linked Genes in Deceased Neonates and Surviving Cloned Female Piglets. Mol Reprod Dev 75:265-73.

55. Everts RE, Chavatte-Palmer P, Razzak A, Hue I, Green CA, Oliveira R, Vignon X, Rodriguez-Zas SL, Tian XC, Yang X, Renard JP, Lewin HA. 2008. Aberrant gene expression patterns in placentomes are associated with phenotypically normal and abnormal cattle cloned by somatic cell nuclear transfer. Physiol Genomics 33:65-77.

56. Li C, Bin YF, Tian XC#, Curchoe C, Yang L, Feng DY, Jiang QY, O’Neill M, Zhang SQ. 2008. Genetic imprinting of H19 and IGF2 in domestic pigs (Sus scrofa). Animal Biotech 19:22-7.

57. Lonergan P, Evans AC, Boland E, Rizos D, Fair T, Duffy P, Sung LY, Du F, Chaubal S, Xu J, Yang X, Tian XC*. 2007. Pregnancy and fetal characteristics after transfer of vitrified in vivo and cloned bovine embryos. Theriogenology 68:1128-37.

58 Jiang L, Jobst P, Lai L, Samuel M, Ayares D, Prather RS, Tian XC*. 2007. Expression of Growth-Regulating Imprinted Genes in Cloned Piglets. Cloning Stem Cells 9:97-106.

59. Sung LY, Shen PC, Jeong BS, Xu J, Chang CC, Cheng WT, Wu JS, Lee SN, Broek D, Faber D, Tian XC, Yang X, Du F. 2007. Premature Chromosome Condensation Is Not Essential for Nuclear Reprogramming in Bovine Somatic Cell Nuclear Transfer. Biol Reprod 76:232-240.

60. Chaubal SA, Ferre LB, Molina JA, Faber DC, Bols PEJ, Rezamand R, Tian X, Yang X. 2007. Hormonal treatments for increasing the oocyte and embryo production in an OPU–IVP system. Theriogenology 67:719–728.

61. Chang C-C, Nagy ZP, Abdelmassih R, Liu J-L, Yang X, Tian XC*. 2006. Interactions of the meiotic spindle with mitotic chromosomes in GV mouse oocytes. RMBonline 12:213-221.

62. Xu J, Guo ZQ, Nedambale TL, Zhang JX, Schenk J, Tian XC, Yang X,Du F. 2006. High Developmental Potential of Vitrified Holstein Cattle Embryos Fertilized in Vitro with Sex-sorted Sperm. J Dairy Sci 89:2510-8.

63. Nedambale TL, Du F, Xu J, Chaubal SA, Dinnyes A,Groen W, Faber D, Dobrinsky JR, Yang X, Tian XC. 2006. Prolonging bovine sperm–oocyte incubation in modified medium 199 improves embryo development rate and the viability of vitrified blastocysts.  Theriogenology 66:1951-1960.

64. Suteevun T, Parnpai R, Smith SL, Chang CC, Muenthaisong S, Tian XC*. 2006. Epigenetic characteristics of cloned and in vitro fertilized swamp buffalo (Bubalus bubalis) embryos. J Anim Sci 84:2065-2071.

65. Chaubal SA, Molina JA, Ohlrichs CA, Ferre LB, Faber DC, Bols PEJ, Riesen J, Tian XC. Yang X. 2006. Different transvaginal ovum pick-up techniques in cows to optimize oocyte retrieval and embryo production over a fixed period of time. Theriogenology 66:1631-1648.

66. Suteevan T, Smith S, Muentaisong S, Yang X, Parnpai R, Tian XC*. 2006. Anomalous mRNA levels of chromatin remodeling genes in swamp buffalo (Bubalus bubalis) cloned embryos. Theriogenology 65:1704-1715.

67. Nedambale TL, Du FL, Yang X, Tian XC*. 2006. Effects of culture media on the development of in vitro-derived bovine embryos, and of β-mercaptoethanol on post-vitrification survival.  Anim Reprod Sci 93:61-75.

68. Du F, Shen P, Xu J, Sung L, Jeong B-S, Nedambale TL, Riesen J, Tian XC, Cheng WTK, Lee S-N, Yang X. 2006. Phytohemagglutinin-cell agglutination agent and embryo vitrification improves the efficiency of somatic cloning in cattle (Bos Taurus). Theriogenology 65: 642-657.

69. Curchoe C, Zhang SQ, Yang L, Bin YF, Zhang XQ, Feng DY, Tian XC*. 2005. Promoter-specific expression of the imprinted Igf2 gene in cattle. Biol Reprod 73:1275-81.

70. Yang L, Chavatte-Palmer P, Kubota C, O’Neill M, Taneja M, Hoagland T, Renard J-P, Yang X, Tian XC*. 2005. Expression of imprinted genes is aberrant in deceased newborn cloned calves and relatively normal in surviving adult clones. Mol Reprod Dev 71:431-8.

71. Vasques LR, Stabellini R, Xue F, Tian XC, Soukoyan M, Pereira LV. 2005. Xist Repression in the absence of Dnmt1 and Dnmt3B. DNA Research 12:373-8.

72. Wang L, Duan E, Sung L-Y, Yang X, Tian XC*. 2005. Generation and characterization of pluripotent putative stem cells from cloned bovine embryos. Biol Reprod 73:149-155.

73. Enright BP, Sung LY, Chang CC, Yang X, Tian XC*. 2005. Methylation and Acetylation Characteristics of Cloned Bovine Embryos from Donor Cells Treated with 5-aza-2'-deoxycytidine. Biol Reprod 72:944-8.

74. Sung L-Y, Du F, Xu J, Chang W, Nedambale TL, Zhang J, Jiang S, Tian XC, Yang X. 2005. The differential requirement of albumin and sodium citrate on the development of in vitro produced bovine embryos. Reprod Nutr Dev 44:551-64.

75. Chang CC, Ma YH, Jacobs S, Tian XC#, Yang X, Rasmussen T. 2005. A maternal store of macroH2A is removed from pronuclei prior to onset of somatic macroH2A expression in preimplantation embryos. Dev Biol 278:367-380.

76. Nedambale TL, Dinnyés A, Groen W, Dobrinsky JR, Tian XC, Yang X. 2004. Comparison on in vitro fertilized bovine embryos cultured in KSOM or SOF and cryopreserved by slow freezing or vitrification. Theriogenology 62: 437-49.

77. Zhang S, Kubota C, Yang L, Zhang Y, Page R, O’Neill M, Yang X, Tian XC*. 2004. Genomic imprinting of H19 in naturally reproduced and cloned cattle. Biol Reprod 71:1540-1544.

78. Liu J-L, Kusakabe H, Chang C-C, Suzuki H, Schmidt DW, Julian M, Pfeffer R, Bormann CL, Tian XC, Yanagimachi R, Yang X. 2004. Freeze-dried sperm fertilization led to term development in rabbits. Biol Reprod 70:1776-1781.

79. Nedambale TL, Dinnyés A, Yang X, Tian XC*. 2004. Bovine blastocyst development in vitro: Timing, sex and viability following vitrification. Biol Reprod 71:1671-1676.

80. Jiang L, Carter DB, Xu J, Yang X, Prather RS, Tian XC*. 2004. Telomere Lengths in Cloned Transgenic Pigs. Biol Reprod 70:1589-1593.

81. Chang CC, Nagy P, Abdelmassih R, Yang X, Tian XC*. 2004. Nuclear and microtubule dynamics of G2/M somatic nuclei during haploidization in germinal vesicle-stage mouse oocytes.  Biol Reprod 70:752-758.

82. Liu J-L, Sung L-Y, Du F, Julian M, Jiang S, Barber M, Xu J, Tian XC, Yang X. 2004. Differential Development of Rabbit Embryos Derived from Parthenogenesis and Nuclear Transfer. Mol Reprod Dev 68:58-64.

83. Nedambale TL, Dinnyes A, Groen W, Dobrinsky JR, Tian XC, Yang X. 2004. Comparison of in vitro fertilized bovine embryos cultured in ISOM or SOFaaci or SOFaaci and cryopreserved by slow freezing or vitrification. Theriogenology 62:437-449.

84. Lee J-W, Tian XC#, Yang X. 2004. Optimization of Parthenogenetic Activation Protocol in Porcine. Mol Reprod Dev 68:51-57.

85. Enright BP, Kubota C, Yang X, Tian XC*. 2003. Epigenetic Characteristics of Donor Cells treated by Trichostatin A or 5-Aza-2’-deoxycytidine and development of embryos cloned from treated donor cells. Biol Reprod 69:896-901.

86. Enright BP, Jeong BS, Yang X, Tian XC*. 2003. Epigenetic Characteristics of bovine Donor Cells for Nuclear Transfer: Levels of Histone Acetylation. Biol Reprod 69:1525-1530.

87. Savage AF, Maull J, Tian XC#, Taneja M, Katz L, Darre M, Yang X. 2003. Behavioral Observations of Adolescent Holstein Heifers Cloned from Adult Somatic Cells. Theriogenology 60:1097-1110.

88. Lee J-W, Tian XC#, Yang X. 2003. Failure of male pronucleus formation is the major cause for the lack of fertilization and embryo development in pig ICSI oocytes. Biol Reprod 68:1341-1347.

89. Lee J-W, Wu S-C, Tian XC#, Barber M, Hoagland T, Riesen J, Yang X. 2003. Production of cloned pigs by whole cell intracytoplasmic microinjection. Biol Reprod 69:995-1001.

90. Govoni KE, Tian XC#, Kazmer GW, Taneja M, Enright B, Rivard AL, Yang X, Zinn SA. 2002. Age-related changes of the somatotropic axis in cloned Holstein calves. Biol Reprod 66:1293-1298.

91. Enright B, Taneja M, Schreiber D, Riesen J, Tian XC#, Fortune JE, Yang X. 2002. Normal onset of puberty in cloned heifers of an aged Holstein cow. Biol Reprod 66:291-296.

92. Du F, Sung L-Y, Tian XC, Yang X. 2002. Differential Cytoplast Requirement for Embryonic and Somatic Cell Nuclear Transfer in Cattle. Mol Reprod Dev 63:183-191.

93. Liu J-L, Sung L-Y, Tian XC#, Yang X. 2002. Hypertonicity-Induced Projections Reflect Cell Polarity in Mouse MII Oocytes: Involvement of Microtubules, Microfilaments, and Chromosomes. Biol Reprod 67:1853-1863.

94. Chen MJ, Kuo Y-H, Tian XC, Chen TT. 2002. Novel biological activities of the fish pro-IGF-I E-peptides: studies on effects of fish pro-IGF-I E-peptide on morphological change, anchorage-dependent cell division, and invasiveness in tumor cells. Gen Comp Endocrinol 126:342-351. 

95. Tian XC, Lonergan P, Jeong BS, Evans AEO, Yang X. 2002. Association of MPF, MAPK and nuclear progression dynamics during activation of young and aged bovine oocytes. Mol Reprod Dev 62:132-138.

96. Tian XC, Chen MJ, Pantschenko AG, Yang TJ, Chen TT. 1999. Recombinant E-peptides of pro-IGF-I have mitogenic activity. Endocrinology 140:3387-3390.

97. Wang PJ, Chabes A, Casagrande R, Tian X, Thelander L, Huffaker TC. 1997. RNR4p, a novel ribonucleotide reductase small subunit protein. Molecular Cellular Biology 17 (10):6114-6121.

98. Rodgers RJ, Vella CA, Young FM, Tian X, Fortune JE. 1995. Levels of cytochrome P450 cholesterol side-chain cleavage enzyme and 3b-hydroxysteroid dehydrogenase during prostaglandin F2a -induced luteal regression in cattle. Reprod Fert Dev 7:1213-1216.

99. Tian X, Berndtson AK, Fortune JE. 1995. Differentiation of bovine preovulatory follicles during the follicular phase is associated with increases in mRNA for cytochrome P-450 side chain cleavage, 3b-hydroxysteroid dehydrogenase and cytochrome P450 17-a hydroxylase, but not cytochrome P-450 aromatase. Endocrinology 136:5102-5110.

100. Tian X, Berndtson AK, Fortune JE. 1993. Changes in levels of messenger ribonucleic acid for cytochrome P450 side-chain cleavage and 3b-hydroxysteorid dehydrogenase during prostaglandin F2a -induced luteolysis in cattle. Biol Reprod 50:349-356.

Invited Reviews/ Book Chapters

1. Tian, XC. 2014. Genomic Imprinting in Farm Animals. Annual Review of Animal BioSciences. (invited). Academic Press.

2. Yong Tang and XC Tian. 2013. Jak/Stat3 and somatic cell reprogramming. JAK-STAT. 2013 2:4, e24935.

3. Qinggang Meng, Zsuzsanna Polgar,  Zsuzsanna Tancos, Xiuchun Cindy Tian, Andras Dinnyes. 2013. Chapter 17. Cloning of Rabbits. In: Principles of Cloning. Academic Press, San Diego, CA.

4. Tian XC, Park J. 2012. Chapter ? Embryonic Gene expression by microarray analysis. In. Encyclopedia of Biotechnology in Agriculture and Food. Heldman DR (ed.), Taylor and Francis Group. (invited and accepted).

5. Tian XC, Marjani SL. 2012. Chapter 2: Epigenetics of cloned pre-implantation embryos of domestic animals. In: Domestic Animal Genetics. Pp. 27-42. Hasan Khatib (ed.). Wiley-Blackwell. A John Wiley & Sons. Ltd Publication, West Sussex UK.

6. Tian XC. 2012. Chapter 11. Bovine epigenetics and epigenome. In: Bovine Genomics. Womack J (ed). Wiley-Blackwell. A John Wiley & Sons. Ltd Publication, West Sussex UK.

7. Dinnyes A, Tian XC, Oback B. 2011. Nuclear transfer for cloning animals. In: Meyers R (ed), Encyclopedia of Molecular Cell Biology and Molecular Medicine, 3rd ed. Weinheim, Germany: Wiley- VCH Verlag, GmbH & Co. KgaA (Oct 10, 2011).

8. Yang X, Guo XM, Wang CY, and Tian XC. 2009. Protocols for Large-Scale Derivation of Cardiomyocytes from Embryonic Stem Cells Chapter 22. In: Essentials of Stem cells Biology.  Lanza R (ed). Elsevier Science, New York New York.

9. Sung LY, Amano T, Smith SL, Tian XC and Yang X. 2009. Somatic Cell Nuclear Transfer and Derivation of Embryonic Stem Cells.  Chapter 1. In: Methods in Bioengineering: Stem Cell Bioengineering.  B Parekkadan and ML Yarmush (eds). Artech House, Norwood MA.

10. Tian XC, Park J, Bruno R, French R, Jiang L, Prather RS. 2009. Altered gene expression in cloned piglets.  Reprod Fertil Dev 21:60-6.

11. Marjani SL, Le Bourhis D, Vignon X, Heyman Y, Everts RE, Rodriguez-Zas SL, Lewin HA, Renard JP, Yang X, Tian XC. 2009. Embryonic gene expression profiling using microarray analysis. Reprod Fertil Dev 21:22-30.

12. Chang CC, Sung LY, Amano T, Tian XC, Yang X, Nagy ZP. 2009. Nuclear transfer and oocyte cryopreservation.  Reprod Fertil Dev 21:37-44.

13. Dinnyes A, XC Tian, X Yang. 2008. Epigenetic regulation of fetal development in nuclear transfer animal models (presented in the 16th International Congress on Animal Reproduction, and a special issue of the Reproduction in Domestic Animals) Reprod Dom Anim 43: Suppl 2: 302-309.

14. Niemann H, Tian XC, King WA, Lee RS. 2008. Epigenetic reprogramming in embryonic and foetal development upon somatic cell nuclear transfer cloning. Reproduction. 135:151-63.

15. Roach M, Wang L,Yang X and Tian XC. 2006. Bovine Embryonic Stem Cells.  In: Methods in Enzymology: Embryonic Stem Cells.  Lanza R (ed). Vol 418:21-37. Academic Press, Elsevier, San Diego, CA.

16. Guo XM, Wang CY, Tian XC, Yang X. 2006. Engineering cardiac tissue from embryonic stem cells. In: Methods in Enzymology: Embryonic Stem Cells.  Lanza R (ed). Vol 420:316-38.  Academic Press, Elsevier, San Diego, CA.

17. Yang X., Wang CY, Guo XM and Tian XC. 2006. Cardiomyocytes.  In: Methods in Enzymology: Embryonic Stem Cells.  Lanza R (ed). Vol 418:267-283. Academic Press, Elsevier, San Diego, CA.

18. Tian XC, Smith SL, Zhang SQ, Kubota C, Curchoe C, Xue F, Yang L, Du F, Sung L-Y, Yang X. 2006. Nuclear reprogramming by somatic cell nuclear transfer – the cattle story. In: Reproduction in Domestic Ruminants VI. Pp. 327-340. Juengel JL, Murray JF and Smith MF (eds). Nottingham University Press, Nottingham, UK.

19. Tian XC. 2005. Modification of donors cells in somatic cell nuclear transfer. Proceedings of the 2nd Asian Reproductive Biotechnology Conference. Pp. 68-76. Bangkok, Thailand, Nov 2-7, 2005.

20. Suteevun T, Smith SL, Muenthaisong, S, Yang X, Parnpai R, Tian XC. 2005. Expression of chromatin remodeling genes in cloned and IVF swamp buffalo embryos. Proceedings of the 2nd Asian Reproductive Biotechnology Conference. Pp. 97-99. Bangkok, Thailand, Nov 2-7, 2005.

21. Dinnyes A, Xu J, and Oback B, Tian XC. 2004. Nuclear transfer for cloning animals. In: Encyclopedia of Molecular Cell Biology and Molecular Medicine. Meyers R (ed), 2nd ed. Weinheim, Germany: Wiley- VCH Verlag, GmbH & Co. KgaA.

22. Yang, X, Tian XC, W Fodor. 2004. Cattle call for gene targeting. Nature Genetic 36: 671-672.

23. Tian XC. 2004. Reprogramming of epigenetic inheritance by somatic cell nuclear transfer. RBMonline 8:501-508.

24. Tian XC, Kubota C, Enright B, Yang X. 2003. Cloning animals by somatic cell nuclear transfer – biological factors. Reprod Biol Endocrinol 1:98-104.

25. Liu L, Deng M, Yang X, Tian XC. 2002. Chapter 17: Activation of mammalian oocytes: principles and practice. Pp. 319-345. In: Introduction to Mammalian Reproduction, Tulsiani D (ed), Kluwer Academic Publishers, Norwell MA.

26. Dinnyes A, Yang X, Tian XC. 2002. Chapter 17: Cloning of Rabbits. In: Principles of Cloning. pp. 343-366. Cibelli JB, Lanza RP, Campbell KHS, West MD (eds). Academic Press, San Diego, CA.

27. Tian XC. 2002. Multiple Hormones From A Single Polypeptide By Post-translational Modifications - An Example By IGF-I E-peptides. Recent Res Dev Endocrinol 3:359-368.

28. Kubota C, Yang X, Tian XC. 2001. Cloning of aged animals - a medical model for tissue and organ regeneration. Trends Cardio Med 11: 313-317.

29. Yang X, Tian XC. 2000. Cloning adult animals-what is the significance? Cloning 2: 117-122.

30. Yang X, Dai Y, Wang B, Tian XC. 2000. Transgenic farm animals: applications in agriculture and biomedicine. Pp. 269-292. In: Biotechnology Annual Review, vol 5. El-Gewely MR (ed), Elsevier Science B.V., Amsterdam, The Netherlands.

31. Yang X, Tian XC. 1998. Life on the bio-pharm: therapeutic proteins from transgenic organisms. Molecular Medicine Today, 4: 424-425.

32. MacCalman CD, Omigobodun A, Fortune JE, Furth EE, Coutifaris C, Strauss JF III, Tian XC. 1996. Novel cell adhesion molecules: Roles in implantation? Pp. 138-154. In: The Endometrium as a Target for contraception. Beier HM, Harper MJK, Chwalisz K (eds), Spring Verlag, Berlin, Germany.

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