John A. McCracken

Professor-in-Residence

 Contact

 
   Phone: (860) 486-6197

   Email: John.McCracken@UConn.edu

   Office: George White Bldg., Room 004C







"UConn Animal Science Research Leads To First Baby Born From a Human Uterine Transplant"

Background

Education

  • BVMS (Vet Science), University of Glasgow, 1958
  • MRCVS (Vet Surgery), Royal College of Veterinary Surgeons, 1958
  • Ph.D. (Endocrinology), University of Glasgow, 1962
  • Post-Doc (Steroid Biochem), University of Birmingham Medical School, 1964

Dr. McCracken was born in Cathcart a suburb of Glasgow, Scotland, but grew up in Troon in the county of Ayrshire, noted for its golf courses such as Royal Troon and Turnberry, not to mention its Ayrshire cattle and Clydesdale horses. He was interested in a career in either Agriculture or Medicine, but as a compromise he chose to enroll in Veterinary Medicine at Glasgow University. After graduating in 1958, he served as a House Surgeon at the Veterinary Hospital in Bearsden, Glasgow. This invaluable experience enabled him to develop skills in surgery and clinical medicine as well as to gain insights into ongoing research. At the end of that year, Sir William Weipers, Dean of the School, convinced John to pursue a Research Fellowship supported by the Scottish Milk Marketing Board. With considerable trepidation, John accepted this directional change in his career. Weipers must have seen in John some hidden talent of which he was completely unaware! John elected to spend the first year of the Fellowship at the University of Cambridge,UK to learn the intricacies of steroid hormone measurement. On returning to Glasgow, John completed his Ph.D. dissertation which included the discovery of progesterone in cow’s milk, later developed into a simple bovine early pregnancy test that is still widely used today. For post-doctoral work, his first choice was the Worcester Foundation for Experimental Biology, in Shrewsbury, Massachusetts, then “Mecca” for the steroid biochemist. When Weipers told John that the post-doctoral fellowship was confined to the UK, John secured a position with the leading light in the steroid field in the UK, Professor Ian Bush at the University of Birmingham Medical School, UK. After two years (1963 & 1964), Bush called John into his office to say that he was immigrating to the United States to a place called the Worcester Foundation for Experimental Biology and would John like to join Bush and his group there! Needless to say, John tactfully accepted Bush’s offer but neglected to mention that the Worcester Foundation had been his original first choice! My exodus with Bush and colleagues to the US gave birth to the term “Brain Drain” and indirectly improved funding for science in the U.K. It was not until many years later that John told Professor Bush that his lab had actually been his second choice for post doctoral studies so that he was only number two and we both had a good laugh! Little did Dr. McCracken know that, with a year off in 1970 as a Visiting Professor at Cornell University with Professor William Hansel, he was to spend the next 34 years at the Worcester Foundation.

 

Academics

Area of Interest / Research Accomplishments:

After arriving at the Worcester Foundation, Gregory Pincus, the co-founder of the Institute and of birth control pill fame, guided John into the study of the inter-relationships of steroid hormones and prostaglandins. This study culminated 1972, in collaboration with Bengt Samuelsson, a Nobel Laureate in Sweden, with the identification of prostaglandin-F2a as the long sought luteolytic hormone that controls the ovarian cycle in most mammals. This work was published in Nature with the capsular subheading: Prostaglandin F2a is released from the uterus of the sheep in a cyclic fashion, acts primarily in a local manner on the ovary via a counter-current mechanism, and is responsible for the periodic regression of the corpus luteum in this species. We published a paper in 2010 showing that the countercurrent transfer of PGF2a in the utero-ovarian vascular pedicle is controlled by the prostaglandin transporter protein. The discoveryand identification of the luteolytic hormone PGF2a proved to be a major breakthrough in the livestock industry where PGF2a or its synthetic analogs are now used routinely to regulate the breeding of domestic animals. Further work revealed that estrogen and progesterone controlled the release of the uterine luteolytic hormone, PGF2a, via the induction of receptors for oxytocin in the endometrium. At the end of the cycle, intermittent releases of oxytocin from the posterior pituitary, as well as from the corpus luteum itself, interacting with the newly formed endometrial receptors for oxytocin, invoke the pulsatile release of PGF2a from the uterus. Moreover,we recently found that a minimum of five pulses of PGF2a is required to consistently cause complete regression of the corpus luteum and hence the induction of a new ovarian cycle. It was also determined that, in the event of pregnancy, the induction of endometrial receptors for oxytocin failed to occur, thus preventing the pulsatile release of PGF2a from the uterus, and thereby allowing the corpus luteum to survive and maintain the pregnancy. Later work by others showed that the blockade of endometrial oxytocin receptors in early pregnancy is due to the secretion of interferon-tau by the developing blastocyst. Other work with prostaglandins included the discovery, with Robert Skarnes an immunologist also at the Worcester Foundation, that fever induced by bacterial endotoxin is caused by the generation in the systemic circulation of prostaglandin E2 which diffuses into the thermoregulatory center in the brain and evokes the first wave of fever. Recent work by others has shown that the second more sustained wave of fever is also caused by prostaglandin-E2, but this time induced locally within the thermoregulatory center in the brain. These findings now explain why both waves of fever are prevented by prostaglandin blocking drugs such as aspirin and acetominaphen (Tylenol).

In 1998, Dr. McCracken retired from the Worcester Foundation as Principal Scientist Emeritus at the time that the Institute was being disbanded. He was then appointed as Professor-in-Residence in the Department of Animal Science at the University of Connecticut where his research has focused on molecular aspects of prostaglandin action, in particular on the molecular mechanism of action of PGF2a in causing regression of the corpus luteum. This research has involved collaboration with Dr. Paul Tsang in the Department of Animal Science at UNH, as well as several members of the Department of Animal Science at UConn. Current work has shown that one of the earliest changes so far reported in the luteolytic action of PGF2a in vivo, is the dramatic change in protein regulators of the extracellular matrix of the corpus luteum. The overall objective of the study is to obtain a detailed picture of the dynamic in vivo temporal changes in a variety of other proteins in the corpus luteum throughout the luteolytic process. Such studies on the growth and regression of the corpus luteum are clearly indicated, not only for understanding basic reproductive processes, but also because the mechanisms controlling the function of this transient gland have important implications for understanding the basis for controlled and uncontrolled growth and regression of other tissues. . In other studies, Dr. McCracken formed a collaboration with Dr. Joe Arosh at Texas A&M University to study the role of the prostaglandin transporter (PGT) protein in the countercurrent transfer of PGF2a within the utero-ovarian plexus (UOP) which permits PGF2a to reach the ovary directly thus avoiding catabolism in the pulmonary circulation. We have shown recently that the PGT protein is highly expressed in the UOP at the time of luteolysis and that the blockade of the PGT protein prevents the transfer of PGF2a within the UOP. Other work with Dr. Arosh has shown that in early pregnancy, there is an up-regulation of PGE2 synthesis and signaling, both in the uterus and CL, which has a luteo-protective effect during the establishment of pregnancy in sheep. In 2006, Dr. McCracken collaborated with a group in Sweden headed by Professor Mats Brännström who’s long term goal was to transplant the human uterus in order to ameliorate certain types of infertilty in women. This collaboration both here at UConn and in Sweden resulted in a uterine transplantation procedure in sheep which served as an excellent model for uterine transplantation in women. On September 20th, 2012, the first mother-daughter uterine transplants in two women was announced by Professor Brännström and his team in Sweden. It is quite remarkable to think that the recipient daughters may eventually have their own children delivered from the same uterus from which they themselves were born!  More recently in 2014, Mats Brännström announced the successful birth of a baby boy from one of his uterine transplant women and several more births have followed, surely the culmination of Mats Brännström's long term endeavor to sucessfully transplant the human uterus.  Professor Brännström recently told me that the success of uterine transplantation in sheep was essential for him to continue his studies in the Baboon which later resulted in his recent success with uterine transplantation in women. Over the years, Dr. McCracken’s work has been supported by competitive grants from NSF, NIH, and most recently by two competitive grants from the USDA and a competitive Internal Research Award from the UConn Foundation.


If anyone would like to learn more about the luteolytic hormone or prostaglandins and leukotrienes in general, John will be glad to send copies of recent textbook chapters on these topics to them.

Positions Held:

1958-1959

House Surgeon, Veterinary Hospital, University of Glasgow (Clinical Medicine and Surgery).

1959-1960

Visiting Research Fellow, University of Cambridge (Dr. R.V. Short, Biochem & Physiol)

1960-1963

Research Fellow, University of Glasgow (Progesterone Metabolism - Professor Sir William Weipers)

1963 & 1964

Research Fellow, University of Birmingham, (Prof. Ian E. Bush - Steroid Biochemistry)

1964-1970

Staff Scientist, Worcester Foundation (Steroid Biochemistry, Endocrinology)

1969-1970

Visiting Professor, Cornell University, (Prof. William Hansel - taught course in Steroid Biochem).

1970-1992

Senior Scientist Worcester Foundation

1976-1986

Affiliate Scientist, New England Regional Primate Res Center, Southboro, MA

1977-1998

Research Professor of Physiology, U.Mass. Medical School, Worcester, MA

1992-1997

Principal Scientist, Worcester Foundation

    
1998-

Principal Scientist Emeritus, Worcester Foundation

    
1998-  

Professor-in-Residence, University of Connecticut, Storrs, CT

Honors/Activities:

  • Member, Biochemical Endocrinology Study Section, NIH, 1979-1983
  • Opponent in Ph.D. Thesis Defense, University of Gothenburg, Sweden for Candidate Arnie Bendz, M.D., "Evidence for a counter-current system in the human adnexa", March 1982
  • Member, Publications Committee, Society for the Study of Reproduction, 1983-1984
  • Member, Reproductive Endocrinology Study Section, NIH, 1984-1988
  • Member, Review Committee, McMaster Reproductive Biology Program, February 1985
  • Member, Steering Committee, Worcester Consortium Ph.D. Program, 1986-1996
  • Opponent in Ph.D. Thesis Defense, University of Uppsala, Sweden for Ph.D. candidate Dr. Samar Basu, "Endometrial prostaglandin synthesis", September 1988
  • Organizer NIH Conference on Autocrine and Paracrine Mechanisms in Reproductive Endocrinology, Worcester Foundation, October 14-16, 1988.
  • Editor, Book entitled, Autocrine and Paracrine Mechanisms in Reproductive Endocrinology, 1989
  • Chairman, Gregory Pincus Medal and Memorial Lecture Committee, 1980-1998 (organized lecture and associated symposia)
  • Chairman and Organizer, New England Endocrinology Conference, 1975, 1980, 1990
  • Member, Awards Committee, Society for the Study of Reproduction, 2001-2003
  • Memorial Lecture for Jack Carlson, SORB Conference, London Ontario, Canada , April, 2005
  • Opponent in Ph.D.Thesis Defense, University of Gothenberg, Sweden for Ph.D. Candidate Pernilla Dahm-Kahler, MD. “The Ovulatory Process: Studies in the Human and the Rabbit”. March, 2007
  • Bowditch Society Lecture, Worcester, MA “Reflections on the 50th Anniversary of the Birth Control Pill”.  October 14th, 2010
  • Medical Grand Rounds Lecture, St Vincent Hospital, University of Massachusetts Medical School. “The Birth Control Pill and Other Milestones in Endocrinology”.  August 18th, 2011

 

Professional Affiliations:

  • American Association for the Advancement of Science
  • Federation of American Scientists
  • Endocrine Society
  • Society for the Study of Reproduction
  • Society for the Study of Fertility
  • New York Academy of Sciences
  • Massachusetts Society for Medical Research

Publications

 Joe A. Arosh, Sakhila Banu, and John A. McCracken (2016) J. Dairy Sci. In press. Novel concepts on the role of prostaglandins on luteal maintenance  and maternal maternal recognition and establishment of pregnancy in ruminants.
   
Joe A. Arosh, JeHoon Lee, Jone A. Stanley, D. Balasubbramanian, Kirthiram K. Sivakumar, Sakhila K. Banu, John A. McCracken. (2016)  Abstr. Accepted 49th Meeting of The SSR. Rescue of the corpus luteum during pregnancy establishment  in sheep is not only due to suppression of endometrial PGF2a pulses but also to increased intraluteal PGE2-EP2/EP4 signaling.

McCracken, J.A. and Arosh, J.A. (2015)  Abstr. Program Directors Meeting ,San Juan, PR. The role of intraluteal prostaglandins in luteolysis and luteal protection in sheep.

Arosh , J.A., Lee, J.H., Stanley, J.A., Banu, S.K., McCracken, J.A.  (2015)  Abstr. #615 48thAnnual Meeting of the SSR. Early pregnancy induces cell survival pathways and inhibits cell apoptosis pathways in the corpus luteum in sheep.

Jehoon Lee, Sakhila K Banu, John A.McCracken and Joe A. Arosh (2015) Reproduction, 151:187-151. Early pregnancy modulates survival and apotosis pathways in the corpus luteum of the sheep.

JeHoon Lee, Jone A. Stanley, John A. McCracken, Sahkila K. Banu, and   Joe A. Arosh (2014) Biol. Reprod.  91: 46-55. Inhibition of ERK1/2 pathways prevents interferon tau inhibition of endometrial expression of oxytocin and estrogen receptors and restores pulsatile release of PGF2a in ruminants.

Lee, J.H., McCracken, J.A., Banu, S.K., Arosh, J. A. (2013). Biol. Reprod. 89: 27-36.  Intrauterine inhibition of prostaglandin transporter protein in ruminants blocks release of  luteolytic PGF2a pulses without suppressing endometrial  expression of estradiol or oxytocin receptors.        
                                                                                                 
McCracken, J.A., Custer, E.E., Schreiber, D.T., Tsang, P.C.W., Keator, C.S. Arosh, J.A. (2012). Prostaglandins and Other Lipid Mediators.  97:90-96. A new in vivo model for luteolysis using systemic pulsatile infusions of PGF2 alpha.

Lee, J.H., McCracken, J.A., Stanley, J.A., Nithy, T.K., Banu, S.K., Arosh, J. A. (2012). Biol. Reprod.  87:1-14 Intraluteal prostaglandin biosynthesis and signaling are selectively directed towards PGF2a during luteolysis but towards PGE2 during the establishment of pregnancy in  sheep.  

McCracken, J.A. (2010) Biol. Reprod. 83: 684-686. Editorial: Reflections on the 50th Anniversary of tthe Birth Control Pill.    
                                                                                                                                                   
Lee, J.H., McCracken, J.A., Banu, S.K., Rodriguez, R., Nithy, T.K., Arosh, J. A. (2010). Endocrinology 151: 3326-3335.  Transport of PGF2a pulses from the uterus to the ovary at the time of luteolysis in ruminants is regulated by prostaglandin transporter-mediated mechanisms. 

Keator, C.S., Custer, E.E., Schreiber, D.T., Hoagland, T.A., McCracken, J.A. (2009). Dom. Anim. Endo.  38: 103-114. Evidence for a potential role of neuropetide Y in ovine corpus luteum function.

Dahm-Kähler, P., Lundmark, C., Enskog, A., Almen-Wranning, C., Racho El-Akouri, R., McCracken, J.A., Brännström, M. (2008).  J. Obstet. Gynec. Res. 34:783-789  Orthoptic autotransplantation of the uterus in the sheep: methodology and early reperfusion events.
                                                                                                                                                               
Keator, C.S., Schreiber, D.T., Hoagland, T.A., and McCracken,J.A. (2008) Dom. Anim. Endo. 35:74-84. Luteolytic and luteotropic effects of nitric oxide in vivo are dose dependent in sheep.

Keator, C.S., Schreiber, D.T., Hoagland, T.A., McCracken,J.A., and Milvae,R.A. (2008).  Dom.Anim. Endo. 34:411-418. Intrauterine infusion of BQ-610, an endothelin type A receptor antagonist, delays luteolysis in dairy heifers.  

McCracken, J.A. (2005). Prostaglandins and Leukotrienes. In: Endocrinology: Basic and Clinical Principles. 2nd Edition, Eds. P. Michael Conn & Shlomo Melmed. Pub:  Humana, Totowa, NJ.

McCracken, J.A. (2004). Prostaglandin F2a: The Luteolytic Hormone. In: The Eicosanoids. Ed: P Curtis-Prior. Pub: John Wiley & Sons, London & New York. Chapter 50, pp525-545.

Towle, T., Tsang, P.C.W., Milvae, R.A., Newbury, M.K., McCracken, J.A. (2002). Biol. Reprod. 66:1515-1521. Dynamic in vivo changes in tissue inhibitors of metalloproteinases-1 and -2, and matrix metalloproteinases -2 and -9, during prostaglandin F2a-induced luteolysis in sheep.

McCracken, J.A. (1999) Dictionary of Reproduction 2: 1055-1062. Eds: E. Knobil and J.D. Neill Pub: Academic Press. Local control systems in reproduction.

McCracken, J.A., Custer, E.E., Lamsa, J.C. (1999) Physiological Reviews 79:263-323. Luteolysis: a neuroendocrine-mediated event.

McCracken, J.A. (1999) Dictionary of Reproduction 2: 1083-1094.  Eds: E.  Knobil and J.D. Neill. Pub:  Academic Press.   Luteolysis.   
                                                                                                       
McCracken, J.A. (1997). In: Endocrinology: Basic and Clinical Principles. Eds. P.M. Conn and S. Melmed. Pub: Humana, Totowa, NJ. Chap 7 pp 101-114. “Prostaglandins and Leukotrienes”.
 
Custer, E.E., Eldering, J.A., Lamsa, J.C., and McCracken, J.A. (1996) Reprod. Dom. Anim. 31: 449-454.  Differential actions of PGF2a on the ovine corpus luteum.
 
McCracken, J.A., Custer, E.E., Eldering, J.A., and Robinson, A.G.(1996) Acta Neurobiol. Exp. 56:819-832.The central oxytocin pulse generator: a pacemaker for the ovarian cycle.
 
McCracken, J.A., E.E. Custer, J. C. Lamsa and A.G. Robinson (1995)   Adv. Exp. Med. Biol. 395:133-154. The central oxytocin pulse generator: a pacemaker for luteolysis.     
                                                   
Custer, E.E., Lamsa, J.C., Eldering, J.A., and McCracken, J.A. (1995)  Adv. Exp. Med. Biol. 395:539-540. In vivo dynamics of oxytocin secretion by the ovine corpus luteum.

Custer, E.E. J.C. Lamsa, J.A. Eldering and J.A. McCracken (1995) Endocrine 3:761-764. Identification of functional high and low affinity states of the prostaglandin F2a receptor in the ovine corpus luteum in vivo and their role in hormone pulsatility.
  
Eldering, J.A., M.G. Nay, L.M. Hoberg, C. Longcope, and J.A. McCracken (1993) Biol. Reprod. 49:809-815. Hormonal regulation of endometrial prostaglandin F2a production during the luteal phase of the rhesus monkey.

Lamsa, J.C., R.A. Cushman, M.G. Nay, and J. A. McCracken (1992) Prostaglandins 43: 165-179.  In vivo desensitization of a high affinity PGF2a receptor in the ovine corpus luteum.

Callard, I.P., Fileti, L.A., Perez,  L.E., Sorbera, L.A., Giannoukos, G., Klosterman,  L.L., Tsang, P., McCracken, J.A.  (1992) American Zoologist  32: 264-275.  Role of the corpus luteum and progesterone in the evolution of vertebrate viviparity.

Silvia, W.J., G.S. Lewis, J.A. McCracken, W.W. Thatcher, and L. Wilson, Jr. (1991)  Biol. Reprod. 45: 655‑663.  Hormonal regulation of uterine secretion of PGF2a during luteolysis in ruminants.

Ivell, R., Hunt, N., Abend, B., Brackman, D., Nollmeyer, J.C. Lamsa, and  J. A. McCracken (1990)  Reprod. Fert. Dev. 2: 703‑711.  Structure and ovarian expression of the oxytocin gene in the sheep.

Eldering, J.A., M.G. Nay, L.M. Hoberg and J.A. McCracken (1990) J. Clin.Endo.Metab.  71: 596‑604. Hormonal regulation of prostaglandin production by rhesus monkey endometrium.

Einer Jensen, N., J.A. McCracken, W. Schramm, and A. Bendz (1989) Acta Physiol Pol. 40:1 11. Counter current transfer in the female adnexa.

Longcope, C., C. Bukowski, W.C. Okulicz, J.A. McCracken, L. Hoberg and H.A. Padykula (1989)  Biol. Reprod. 40: 949‑952.  Estrogen interconversions in the induced cycle in female rhesus monkeys.

Lamsa, J.C., S.J. Kot, J.A. Eldering, M.G. Nay, and J.A. McCracken (1989) Biol.Reprod. 40: 1215 1223.  Prostaglandin F2a stimulated release of ovarian oxytocin in the sheep in vivo: threshold and dose dependency.

Padykula, H.A., L.G. Coles, W.C. Okulicz, S.I. Rappaport, J.A. McCracken, N. W. King, C. Longcope and I. Kaiserman‑Abramof  (1989) Biol. Reprod. 40: 681‑690.  The basalis of the primate endometrium: a bifunctional compartment.

Craig, G. M. and J.A. McCracken (1989) Prostaglandins, Leukotrienes and Essential Fatty Acids 35: 93‑104.  Evidence for involvement of prostaglandins in central adrenergic activity and LH release in sheep.

Longcope, C., C. Bourget, P.A. Meciak, W.C. Okulicz, J.A. McCracken, L.M. Hoberg and H.A. Padykula  (1988) Biol. Reprod. 39: 561‑565. Estrogen dynamics in the female rhesus monkey.

McCracken, J.A., and W. Schramm (1988) In: Prostaglandins: Biology and Chemistry of Prostaglandins and Related Eicosanoids.  Ed: B.Curtis‑Prior. Pub:Churchill‑Livingstone, Edinburgh.  Chapter 34, pp. 425‑462.  "Prostaglandins and corpus luteum regression."

Geuze, H.J., J.W. Slot, K. Yanagibashi, J.A. McCracken, A.L. Schwartz, and P.F. Hall (1987)  Histochemistry,  86: 551‑557.  Immunogold cytochemistry of cytochromes P‑450 in porcine adrenal cortex.  Two enzymes (side chain cleavage and 11‑beta hydroxylase) are co‑localized in the same mitochondria.

Schramm, W., N. Einer‑Jensen, G. Schramm and J.A. McCracken (1986) Biol. Reprod. 34: 671‑680.  Local transfer of oxytocin from the ovarian vein to the ovarian arteries in sheep.

Leavitt, W.W., W.C. Okulicz, W. Schramm, W.F. Robidoux and J.A. McCracken (1985) J. Steroid Biochem. 22: 687‑691.  Estrogen receptor and oxytocin receptor evolution in the ovine uterus following progesterone withdrawal.

Padykula, H.A., L.G. Coles, J.A. McCracken, N.W. King,Jr., C. Longcope and I.R. Kaiserman‑Abramof (1984)  Biol. Reprod. 31: 1103‑1118.  A zonal pattern of cell proliferation and differentiation in the rhesus endometrium during the estrogen surge.

Schramm, W., N. Einer‑Jensen, M.B. Brown and J.A. McCracken (1984) Biol. Reprod. 30: 523‑531.  Effect of four primary prostaglandins and relaxin on blood flow in the ovine endometrium and myometrium.

McCracken, J.A., Schramm, W., Einer‑Jensen,  N.  (1984) Steroids 43: 293‑303.  The structure of steroids and their diffusion through blood vessel walls in a counter current system.

McCracken, J.A. (1984) Res. in Reprod. 16:1‑2.  Update on luteolysis: Receptor regulation of pulsatile secretion of prostaglandin F2a from the uterus.  

McCracken, J.A., Schramm, W., Okulicz, W.C. (1984). Anim. Reprod. Sci. 7:31-55. Hormone receptor control of pulsatile secretion of PGF2a from the ovine uterus during luteolysis and its abrogation during early pregnancy.

Schramm, W., Friesen, H.G., Robertson, H.A., McCracken,  J.A. (1984).  J. Reprod. Fertil. 70: 557‑565.  The effect of ovine placental lactogen on ovarian progesterone and on corpus luteum regression induced by PGF2a.

Schramm, W., Bovaird, L.C., Glew, M.E., Schramm, G., McCracken, J.A. (1983). Prostaglandins 26: 347‑364.  Corpus luteum regression induced by ultra‑low pulses of PGF2a.

Einer‑Jensen, N. and J.A. McCracken (1981).  Endocrinology 109: 685‑691.  The transfer of progesterone in the ovarian pedicle of the sheep.                                                                                   
                       
Skarnes, R.C., Brown, S.K., Hull, S.S. Jr., McCracken, J.A.  (1981) J. Exper.Med. 154: 1212‑1224.  The role of prostaglandin E in the biphasic fever response to endotoxin.
                                             
McCracken, J.A. (1980). In: Adv Prostagl. Thromb. Res. 8:1329-1344. Hormone receptor control of prostaglandin secretion by the ovine uterus.

Skarnes, R.C., McCracken,  J.A.  (1980). Microbiology-1980, 162-165. The mediating role of prostaglandin E in endotoxin fever.

McCracken, J.A., Einer‑Jensen, N., Fried, J. (1979) Adv. Exptl. Med. Biol. 112: 577‑601.  Prostaglandin F2a and its 13‑dehydro analogs: comparative luteolytic effects in vivo.  
          
McCracken, J.A., Gammal, L.M., Glew, M.E., Underwood, L.F. (1978). Endocrinology 102: A440.  Hormone receptor control of PGF2a secretion from the ovine uterus.

Einer‑Jensen, N., McCracken, J.A.  (1977).Prostaglandins 13: 763‑775.  The effect of prostaglandin F2a on the counter current transfer of 85Krypton in the ovarian pedicle of the sheep.

McCracken, J.A.,  Fried, J., Lee, M‑S., Yoshikawa, Y., Mammato, D.C.  (1977)  In: Biochem.Aspects Prostagl. Thrombox. Res.  Ed:  N. Kharasch and J. Fried.  Pub:  Academic Press, New York. 212‑242.  Synthesis and biological studies of luteolytic 13‑dehydroprostaglandins.

Roberts, J.S., J.A. McCracken, J. Gavagan and M.S. Soloff (1976).  Endocrinology 99: 1107‑1114.  Oxytocin‑stimulated release of prostaglandin F2a from ovine endometrium: correlation with estrous cycle and oxytocin‑receptor binding.       

Roberts, J.S. and J.A. McCracken (1976).  Biol. Reprod. 15: 457‑463.  Does prostaglandin F2a released from the uterus by oxytocin mediate the uterotonic action of oxytocin?

Roberts, J.S., Barcikowski, B., Wilson, L., Skarnes, R.C., McCracken, J.A. (1975). J. Steroid Biochem. 6:1091-1097. Hormonal and related factors affecting the release of PGF2a from the uterus.

Barcikowski, B., Carlson, J.C., Wilson, L., McCracken, J.A. (1974). Endocrinology 95:1340-1349. The effect of endogenous and exogenous estradiol-17B on the release of PGF2a from the ovine uterus.

McCracken, J.A., Barcikowski, B., Carlson, J.C., Green, K., Samuelsson, B. (1973). Adv. Biosci. 9:599-624. The physiological role of prostaglandin F2a in regression of the corpus luteum.

McCracken, J.A.  (1973)  Prostaglandins 3: 691‑695. Commentary.

McCracken, J.A.  (1972) Prostaglandins in Fertility Control 2: 234‑258.  WHO Meeting Stockholm, Sweden.  The role of prostaglandins in luteal regression.

McCracken, J.A., Carlson, J.C., Glew, M.E., Goding, J.R., Baird, D.T., Green, K., Samuelsson, B. (1972). Nature New Biol. (London) 238:129-134. Prostaglandin F2a identified as a luteolytic hormone in sheep.

McCracken, J.A. (1971) Ann. NY Acad. Sci. 180:456-472.  Prostaglandin F2a and corpus luteum regression.

McCracken, J.A., Baird, D.T., Goding, J.R. (1971). Rec. Progr.Hor. Res. 27:537-582. Factors affecting the secretion of steroids from the transplanted ovary of the sheep.

McCracken, J.A., Glew, M.E., Scaramuzzi, R. (1970). J. Clin. Endo. Metab. 30:544-546. Corpus luteum regression induced by prostaglandin F2a.

Hobbies/Non-Academic Interests

John’s hobbies include tennis, flyfishing and the collection of rare single malts. He tries to visit Scotland every other year and has a penchant for staying in castles, which have included Brodick, Barcaldine, and Fyvie, famous for its Bonnie Lass! He also has a long standing interest in conservation and is a life member of the Sierra Club, the Appalachian Mountain Club, Massachusetts Audubon Society, Trout Unlimited, and the National Trust for Scotland.

 

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